All samples submitted must be clearly labeled (waterproof). The labeling must contain the name of the sample owner and an identification number or code of the sample. In case of samples separated by gel electrophoresis, please provide a scan of the stained gel where the submitted bands/spots are labeled.

In order to successfully analyze your samples, we ask you to complete the form bellow. Please provide as many information as available about the nature of the samples and about your requirements, this contributes greatly to a successful analysis.

Please consider, that not every sample will lead to a successful analysis. Common problems we encounter include:

• contaminants - principally human or sheep keratins and polymers

• wide discrepancy between "expected" and actual amounts of protein

• use of inappropriate staining (see sample preparation guidelines)

• diffuse protein bands

Availability - Pricing

The usage of this facility is not restricted only to the university institutes on the Campus and Intercell, but is open in principle also for other customers. However, it must be stated, that samples submitted from the Campus always have priority, and samples submitted by other institutions will only be analyzed according to the capacity.Pricing depends on the number of samples and the type of the analysis, please contact us for further information.

Sample submission form (.rtf)

1. Contact information of the group / project leader:

• Name:

• Telephone:

• e-mail:

• Invoice to:

signature of the group / project leader

2. Contact information of the sample owner

• Name:

• Telephone:

• e-mail:

3. Date of the submission:

4. Sample description:

• Identification name:

• What form is the sample in:

• Estimated amount of protein (sample amount needed is around one picomole - a clear Coomassie or silver stained gel band / spot):

• Is the protein labeled with any radioactive tracer ? If so, which isotope and what specific activity ?

• What is the exact gel used ?

• What buffers were used to run the gel?:

• What stain was used to visualize the protein, and how was the gel treated and stored following the staining ?

For silver staining of gels, please use the protocol we recommend. Excised bands (in Eppendorf tubes) should be submitted slightly wet.

5. Sample background information

• What exactly did you do to arrive at a sample ready to load on a gel?

• N.B. Please provide full details of all isolation procedures used, with special reference to detergents, salts, immuno-affinity reagents (all antibodies are proteins).

• Which organism is the protein isolated from ?

• Are any (posttranslational) modifications or variants known / expected?

• Can the full sequence of the protein be found in a data base? (accession number)

• Please include any data you already have concerning molecular weight, pI, and biochemical or biological function.

6. Additional requirements (blanks)

It is really helpful to provide a blank, run under the same conditions but with no protein added. (Simply cut out a blank area near the sample.)

©ECs February 2007